Uses antibodies against tumor antigens to measure levels of protein
Utilizes fluorescent probes that bind to specific DNA sequences to identify chromosomal abnormalities
High-throughput method used to determine the nucleotide sequence of entire genomes or a broad panel of target genes
Used to amplify specific targets and detect alternative variants that lead to exon skipping
Determines the nucleotide sequence of DNA and is used when analyzing small regions of the DNA on a limited number of samples or genomic targets
ex14=exon 14; FDA=Food and Drug Administration; FISH=fluorescence in situ hybridization; IHC=immunohistochemistry; MET=mesenchymal-epithelial transition; NGS=next-generation sequencing; NSCLC=non-small cell lung cancer; RT-PCR=reverse transcription polymerase chain reaction.
*Turnaround times are approximate and may vary for individual tests/assays.
FFPE=formalin-fixed paraffin-embedded; H&E=hematoxylin and eosin; MET=mesenchymal-epithelial transition.
FDA=Food and Drug Administration; IHC=immunohistochemistry; MET=mesenchymal-epithelial transition.
*Turnaround times are approximate and may vary for individual tests/assays.
CEP7=centromere 7; FFPE=formalin-fixed paraffin-embedded; FISH=fluorescence in situ hybridization; MET=mesenchymal-epithelial transition.
CEP7=centromere 7; Chr=chromosome; FDA=Food and Drug Administration; FISH=fluorescence in situ hybridization; MET=mesenchymal-epithelial transition; WT=wildtype.
*Turnaround times are approximate and may vary for individual tests/assays.
ex14=exon 14; FFPE=formalin-fixed paraffin-embedded; MET=mesenchymal-epithelial transition; NGS=next-generation sequencing.
ex14=exon 14; MET=mesenchymal-epithelial transition; NGS=next-generation sequencing; NSCLC=non-small cell lung cancer.
*Turnaround times are approximate and may vary for individual tests/assays.
FFPE=formalin-fixed paraffin-embedded; MET=mesenchymal-epithelial transition; NGS=next-generation sequencing.
‡The definition of high-level MET amplification is evolving and may differ according to the assay used for testing. Referenced with permission from the NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines®) for Non-Small Cell lung Cancer V.7.2024. © National Comprehensive Cancer Network, Inc. 2024. All rights reserved. Accessed June 26, 2024. To view the most recent and complete version of the guideline, go online to NCCN.org. NCCN makes no warranties of any kind whatsoever regarding their content, use or application and disclaims any responsibility for their application or use in any way.
CAPZA2=capping actin protein of muscle z-line subunit alpha 2; Chr=chromosome; FDA=Food and Drug Administration; MET=mesenchymal-epithelial transition; NGS=next-generation sequencing.
c-Met protein overexpression and MET amplification are emerging biomarkers and in clinical research as potential therapeutic targets. There are no FDA-approved tests for c-Met protein overexpression or MET amplification. The shown images are for illustrative purposes only. †Method results should be interpreted by a qualified pathologist in conjunction with histologic examination, relevant clinical information, and proper controls.